S.N. Research Center,

Ambernath, India. Two representative gram positive Bacillus subtilis and Staphylococcus aureus and two representative gram negative Escherichia coli and Pseudomonas aeruginosa were procured. The microbial strains were maintained onto agar slants at 4°C. Mueller-Hinton agar plates were then prepared and spread plated with bacteria. Well-diffusion method was employed for carrying out the antimicrobial activity. Four wells were bored with sterile cork borer. Wells were labeled, respectively, for distilled water as negative control, ciprofloxacin (1mM) as positive control, and other two equivalent concentrations of test samples, C-dots and Cipro@C-dots conjugate, in each keeping C-dots #buy Rigosertib keyword# concentration constant in both the samples. 3. Results and Discussion GA is extremely branched

arabinogalactan polysaccharide [19]. Due to the very high content of branched carbon and proteins, it could act as versatile raw material for the synthesis of highly fluorescent C-dots by microwave assisted carbonization. Color of GA Inhibitors,research,lifescience,medical (pale yellow) got transformed to wine red after heating for 5min under the influence of EtOH and NaOH as surface passivation agents. This was color marker for synthesis of C-dots as per previous Inhibitors,research,lifescience,medical studies [20]. Under UV light (λ = 365nm), turbid green fluorescence was observed, which may be due to presence of partially burnt carbonaceous materials along with graphene oxide (GO). Nanoparticulate systems never possess monodispersed particles by virtue of strange quantum mechanical attributes and thermodynamics at nanoscale [21]. Therefore, Inhibitors,research,lifescience,medical for efficient application of C-dots, its separation became mandatory using SDGC. SDGC separates nanoparticles based on their hydrodynamic properties. Due to negligible impact of gravity, inertia, and dominant thermal energies, separation of ultrasmall particles such as C-dots is not possible by simple centrifugation techniques [22]. Fractions are separated based on their densities with respect to sucrose gradient. Three discrete bands were seen with different fluorescence Inhibitors,research,lifescience,medical intensities as shown in Figure

1. For systematic discussions on optical as well as morphological properties of isolated bands, B1, B2, and B3 are considered separately (SI S1 for quantum yield values). Figure 1 Separation of C-dots using SDGC. (a) Separated Urease bands under ambient light and (b) 250nm excitation UV lamp. Upper and lower panels show color of the fractions under normal light and UV light, respectively. UV-Vis analysis of B1 shows a sharp peak at 243 and a shoulder at 267nm (Figure 2(a)). Presence of dual peak is signature marker of C-dots as per earlier studies [23]. Origin of intense UV peaks is speculated due to π electron transitions in graphene quantum dots (GQDs) containing oxygen as functional groups. Absorbance at 216nm is due to π → π* electron transition of C=C and 273nm is due to n → π* of carbonyl groups [24].