Biochem Biophys Res Commun 1960, 3:654–659.PubMedCrossRef 28. Grass G, Rensing C, Solioz M: Metallic copper as an antimicrobial surface. Appl Environ Microbiol 2011, 77(5):1541–1547.PubMedCrossRefPubMedCentral 29. Gupta SD, Wu HC, Rick PD: A Salmonella typhimurium genetic locus which confers copper tolerance on copper-sensitive mutants of Escherichia coli . J Bacteriol 1997, 179(16):4977–4984.PubMedPubMedCentral 30. Nataro JP, Seriwatana J, Fasano A, Maneval DR, Guers LD, Noriega F, Dubovsky F, Levine MM, Morris JG: Identification and cloning of a novel plasmid-encoded enterotoxin of enteroinvasive Escherichia coli and Shigella strains. MM-102 research buy Infect Immun 1995,

63(12):4721–4728.PubMedPubMedCentral 31. Badger JL, Wass CA, Weissman SJ, Kim KS: Application of signature-tagged Epacadostat mutagenesis for identification of Escherichia coli K1 genes that contribute to invasion of human brain microvascular endothelial cells. Infect Immun 2000, 68(9):5056–5061.PubMedCrossRefPubMedCentral 32. Huang

SH, Wass C, Fu Q, Prasadarao NV, Stins M, Kim KS: Escherichia coli invasion of brain microvascular endothelial cells in vitro and in vivo selleck : molecular cloning and characterization of invasion gene ibe10 . Infec Immun 1995, 63(11):4470–4475. 33. Sambrook J, Russell DW: Molecular cloning: a laboratory manual. In, Volume 1. 4th edition. New York: Cold Spring Harbor Laboratory Press; 2012. 34. Aziz R, Bartels D, Best A, DeJongh M, Disz T, Edwards the R, Formsma K, Gerdes S, Glass E, Kubal M, Meyer F, Olsen G, Olson R, Osterman A, Overbeek R, McNeil L, Paarmann D, Paczian T, Parrello B, Pusch G, Reich C, Stevens R, Vassieva O, Vonstein V, Wilke A, Zagnitko O: The RAST server: rapid annotations using subsystems technology. BMC Genomics 2008, 9(1):75. 35. Darling ACE, Mau B, Blattner FR, Perna NT: Mauve: multiple

alignment of conserved genomic sequence with rearrangements. Genome Res 2004, 14(7):1394–1403.PubMedCrossRefPubMedCentral 36. Hall BG: Building phylogenetic trees from molecular data with MEGA. Mol Biol Evol 2013, 30(5):1229–1235.PubMedCrossRef 37. Saitou N, Nei M: The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987, 4(4):406–425.PubMed 38. Johnson JR, Stell AL: Extended virulence genotypes of Escherichia coli strains from patients with urosepsis in relation to phylogeny and host compromise. J Infect Dis 2000, 181(1):261–272.PubMedCrossRef 39. Datsenko KA, Wanner BL: One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci 2000, 97(12):6640–6645.PubMedCrossRefPubMedCentral 40. El-Mansi M, Anderson KJ, Inche CA, Knowles LK, Platt DJ: Isolation and curing of the Klebsiella pneumoniae large indigenous plasmid using sodium dodecyl sulphate. Res Microbiol 2000, 151(3):201–208.PubMedCrossRef 41.