, 2008). EPEC and EHEC O26 strains also play a role as enteropathogens of animals and have been isolated from cattle, sheep,

pigs, goats, rabbits and chicken (Milon et al., 1999; Aktan et al., 2004; Leomil et al., 2005). Humans may become infected with pathogenic E. coli O26 strains on contact with excreta of animals or humans and by ingestion of contaminated foodstuff, and outbreaks of disease with EPEC and EHEC O26 strains have been reported from many different countries (summarized in Jenkins et al., 2008). EPEC and EHEC O26:H11/NM strains were extensively investigated for their virulence attributes and the underlying genes. EHEC O26:H11/NM strains were found to be positive for Stx1, Stx2 or both toxins, and it was this website suggested that the Stx2-expressing subgroup

has evolved more recently (Zhang et al., 2000b; Jenkins et al., 2008). EPEC and EHEC O26:[H11] strains were found to be conserved for the presence of chromosomally Z-VAD-FMK research buy encoded virulence attributes such as the locus of enterocyte effacement (LEE), long polar fimbriae (lpfAO26), an iron repressible protein (irp-2), the yersiniabactin receptor (fyuA) and the porcine-attaching and -effacing protein (paa) gene (An et al., 1999; Trabulsi et al., 2002; Bielaszewska et al., 2005; Leomil et al., 2005; Jenkins et al., 2008). Plasmids encoding EHEC-haemolysin (e-hlyA), catalase peroxidase (katP), and serine protease (espP) are found in most EHEC and in some EPEC O26:[H11] strains (Brunder et al., 1999;

Zhang et al., 2000b; Bielaszewska et al., 2005). EPEC and EHEC O26:H11/NM strains share similar biochemical profiles and are characterized by nonfermentation of rhamnose and dulcitol (RDF−) (Leomil et al., 2005). Correspondingly, EPEC and EHEC O26:[H11] strains Liothyronine Sodium were found to be genetically closely related as demonstrated by multilocus enzyme electrophoresis (MLEE) (Whittam et al., 1993). More recently, another group of atypical EPEC O26:NM strains was described that differs from the EPEC/EHEC O26:H11/NM group strains by fermentation of rhamnose and dulcitol (RDF+), and by the XbaI pulsed-field gel electrophoresis (PFGE) genotype. Multilocus sequence typing (MLST) of ‘housekeeping genes’ present in strains belonging to both groups revealed high genetic similarity, and differences between the RDF− and RDF+ groups were found only in the arcA gene sequence (Leomil et al., 2005). Another characteristic trait of strains belonging to the O26:NM RDF+ group is the presence of large-size conjugative plasmids encoding α-haemolysin (α-hlyA) (Leomil et al., 2005; Burgos et al., 2009). In contrast, plasmid encoded e-hlyA, katP and espP genes are absent in these strains (Leomil et al., 2005). A third lineage of E. coli O26 strains is represented by the serotype O26:H32. Three O26:H32 strains that were previously investigated were found to be negative for eae and stx genes (Zhang et al., 2000a).