2E). The MMP loss was increased from 6% to 63% in untreated and DQQ treated MOLT-4 cells, respectively (Fig. 2E). We investigate the pathway of apoptosis induced by DQQ in MOLT-4 cells by monitoring the level of different mitochondrial proteins and caspases. Upregulation of Bax and down regulation of Bcl-2 have long been associated with the activation of apoptosis. DQQ inhibit the mitochondrial

anti-apoptotic protein Bcl-2 and induce the translocation of Bax from cytosol to mitochondria and simultaneously released cytochrome c from mitochondria to cytosol, which was associated with mitochondrial membrane potential loss (Fig. 3A, 2E). DQQ drastically reduce the Bcl-2/Bax ratio in MOLT-4 cells from 10 to 0.2 levels (Fig. 3 C). The Bcl-2/Bax ratio has also been found Metformin nmr to play key role in the activation of caspase-3 [25]. Caspase activation is one of the basic events in the process of apoptosis. DQQ significantly induce caspase-3 and -8 levels (4 times) in MOLT-4 cells in a dose dependant manner (Fig. 3B). The caspase activation was further confirmed by western blotting against procaspase-3 and procaspase-8 (Fig. 3A). DQQ significantly alter mitochondrial apoptotic proteins and caspase-8 level that interlinks both the apoptotic pathway and Selleckchem E7080 finally lead to caspase-3 activation and PARP-1 cleavage (Fig. 3A-C). The above data suggest that DQQ

induced apoptosis in MOLT-4 cells via both extrinsic and intrinsic pathways. The role of AKT/mTOR has long been contemplated in the regulation of autophagy and apoptosis. This pathway has been reported as a negative regulator of HSP90 both apoptosis and autophagy [26]. Therefore, it was evident to see the effect of DQQ on the proteins of AKT/mTOR pathway. Western blot analysis

of different proteins of this pathway revealed that DQQ significantly hampered the expression of pAKT, pmTOR and its substrate pP70S6 K in MOLT-4 cells (Fig. 3A). The most significant inhibitory effect was on pmTOR followed by its substrate p70S6 K (Fig. 3A). The mTOR kinase IC50 value of DQQ was found to be 6 nM in a cell free Elisa assay (Fig. 3D). DQQ was found to be a strong mTOR inhibitor and its expression almost negligible, even at low concentration (2 μM). The autophagy induction in cells treated with DQQ was analyzed by acridine orange staining. The results of acridine orange staining revealed that it induced formation of acidic vacuolar organelles (AVO) in MOLT-4 cells, while the number of AVO was negligible in control cells. The number of AVO increased with increasing doses of DQQ (Fig. 4A). Furthermore, western blot analysis of key proteins of autophagy such as beclin1, ATG7, ATG5 and LC3-II revealed that DQQ significantly increased their expression in a dose dependent manner (Fig. 4A). The autophagy induction was further confirmed by LC3 immunofluorescence. The results indicated that DQQ treatment induced dose dependent increase in LC3 fluorescence in MOLT-4 cells (Fig.