A considerable number of specimens need to be even further explored to find out if SNCG protein expression can be a pre dictive biomarker for evaluation of pre surgical procedure and survival of prostate cancer sufferers. Conclusions In summary, SNCG is aberrantly expressed in PCa and is connected with its malignant progression.
Our information supply proof that SNCG protein expression may possibly serve like a biomarker for assessment of biopsies selleck to pre dict a substantial possibility of prostate cancer progression and me tastasis. SNCG is regulated by androgen, interacts with AR protein and influences AR target gene PSA expression by enhancing androgen induced AR transcriptional ac tivity, indicating that the functional roles of SNCG in PCa may very well be associated for the androgenAR signaling path way.
Since SNCG is concerned within a wide range of biological actions of hop over to these guys PCa which includes cellular proliferation, migra tion and invasion in vitro too as tumorigenesis in vivo, we propose that modulation of SNCG expression may very well be a helpful approach for producing novel biomed ical therapeutics for PCa. The answers on the reviewers one.
It might be preferable to check out an additional AR dependent cell line e. g. CWR22 or use of the other siRNAs to determine a dose response result of SNCG inhibition. We detected the inhibition efficiency of oligo 166 target to SNCG in CWR22 cells, as anticipated, we had observed the inhibition result of SNCG with dose dependent of siSNCG 166 in CWR22 cells. 2.
The invasionmigration assays show a modest inhibition of invasion and migration, thinking of the level of SNCG knockdown, what is the mechanism for this inhibition Is there an inhibition of MMP expression or Rho GTPases for example We supplement the experiment of your relation among inhibition SNCG and MMP working with wild variety LNCaP cells and stable cell line of RFP SNCG LNCaP and siSNCG 166 LNCaP. Through the result we confirmed the expression of SNCG is involving inside the MMP members regulation.
three. The authors looked at the effects of SNCG inhibition and improved expression in LnCap cells. Inhibition of SNCG resulted in decreased proliferation and accumulation during the G1 phase. Vice versa increased SNCG brought about enhanced proliferation, nevertheless the authors tend not to current corresponding information on Cell cycle progression.
Did they not acquire this information Some overexpression experiments have been finished later, right here we primarily show the function of inhibition of SNCG in LNCaP cells. In this way, it avoids the seeming confusion with the entire report construction. 4. The authors carried out quite a few experiments in LNCaP AI cells. They ought to state how these cells assess