(C) 2013 Elsevier B.V. All rights reserved.”
“Objectives: Ghrelin has been implicated in the regulation of gastric growth and functional development, but it is yet to be determined whether and how ghrelin over-expression may modify gastric growth, gastric acid secretion and mRNA expression of other gastric endocrine hormones. 25-day-old mice were injected intra-muscularly with vacant plasmid (VP) or recombinant plasmid expressing secretory ghrelin at the doses of 50 mu g (LG) and 100 mu g (HG).
Results:
Expression of ghrelin mRNA was detected in muscles 15 days post-injection, being most abundant in HG mice. In accordance with the ghrelin expression, gastric weight increased (P < 0.05) in HG mice, compared with VP control group. Significant increase of gastric mucosa H+-K+-ATPase mRNA learn more expression was detected in HG mice compared to VP control group (P < 0.05). Compared with VP mice, gastric somatostatin (SS) mRNA expression decreased in LG and
HG mice (P < 0.05), while gastric gastrin expression had no significant difference.
Conclusions: I.M. injection of plasmid encoding ghrelin improved gastric growth and gastric acid secretion with decreased SS mRNA in weaned mice. Crown Copyright (C) 2013 Published by Elsevier B.V. All rights reserved.”
“Ghrelin is an important gastrointestinal hormone involved in the regulation of feeding Talazoparib in both mammals and fish. In this study, the preproghrelin cDNA sequence was cloning in the gut of Schizothorax prenanti (S. prenanti). The preproghrelin gene, encoding 103-amino acids, was strongly expressed in the gut
and brain using real-time quantitative RT-PCR (qPCR). The S. prenanti preproghrelin was detected in embryonic developmental stages. Further, it was detectable Z-IETD-FMK cell line in unfertilized eggs, suggesting that ghrelin could be classified as maternal mRNA. An experiment was conducted to determine the expression profile of ghrelin during post-feeding and fasting status of the brain and gut. The results revealed a significant postprandial decrease in ghrelin mRNA expression in the gut 6 h post-feeding (hpf) and brain (1.5 and 9 hpf) compared to an unfed control group, indicating that food intake and processing affect the regulation of expression of ghrelin in S. prenanti. The constructed recombinant plasmid pMD-19 T-ghrelin was transformed to Escherichia coli BL21 and induced with IPTG, and the expressed product was identified by SDS-PAGE. The prokaryotic expression vector for ghrelin was constructed successfully, and fusion protein was expressed in E. coli BL21, which laid the foundation for the further study on the function of this protein and its mechanism. Overall, our results provide evidence for a highly conserved structure and biological actions of ghrelin in S. prenanti.