Today’s study seeks to investigate the role of G gp in the transportation of Danshensu across the BBB by observing Danshensu concentration in plasma and brain tissue in mice. Danshensu was obtained from Shandong Luye Pharmaceutical Co., Ltd.. Verapamil was obtained from Shanghai Hefeng Pharmaceutical Co., Ltd.. Naproxen LY364947 was obtained from National Institute for the Get a grip on of Pharmaceutical and Biological Products. Ethyl acetate was obtained from Sinopharm Chemical Reagent Co., Ltd.. Acetonitrile was obtained from Merck. Forty eight male Sprague Dawley rats weighing 220 20 h were supplied by the Experimental Animal Center of Shandong Engineering Research Center Afatinib 439081-18-2 for Natural Drugs, certicate number 20030020. All experimental procedures carried out in this study were done relative to the principles for the Use and Care of Laboratory Animals of Yantai University. The mice were maintained with free usage of food and water on a 12 h light/dark cycle. These were stored Infectious causes of cancer in plastic cages and randomly divided into two teams with 24 animals in each group: the control group and the verapamil group. The rats in the verapamil group were administered intraperitoneally with verapamil at a dose of 20 mg kg1. The subjects in the get a grip on group were treated with the exact same level of normal saline. Ninety minutes later, all rats were handled intravenously with Danshensu by tail vein. At 30 min, 15 min, and 60 min after Danshensu cure, the animals were anesthetized with chloral hydrate and then 5 mL heparinized blood were obtained from abdominal aorta and the rats were perfused with 100 mL of ice cold normal saline each. Mental performance was rapidly taken from the cranium and weighed. Then a mind was homogenized in 4 volumes of 0. 1 mol L1 ice phosphate buer. Three milliliters of ethyl acetate was added into 200 uL of the homogenate. After vortexing for 3 min and centrifuging for 5 min, the supernatants were evaporated to dryness under a gentle nitrogen akt2 inhibitor stream at 40 C. The deposits were resuspended in mobile phase. The blood samples were centrifugated for 10 min and plasma was separated. Plasma was treated as described for brain homogenate supernatants. The chromatographic separation was performed utilizing an Agilent 1100 Series HPLC system built with a vacuum degasser, a quaternary push, an, and a column oven. The chromatographic separation was run on a ODS C18 column. The mobile phase was acetonitrilewater. The pump was operated at a rate of 0. 2 mL min1. Separations were done at the temperature of 20 C. Mass spectrometric detection was done employing a TSQ Quantum tandem mass spectrometer designed with an electrospray ionization source.