There are two main schools

There are two main schools click here of thought on the subject: one holds that lung remodeling is a response to repeated

inflammatory injuries caused by cigarette smoke exposure and represents a trend toward developing abnormal inflammatory reactions to small stimuli (Jeffery, 2001). This point of view accounts for changes in airway structure as an exaggerated healing process by inflammatory cells. Another perspective is that lung remodeling is a product of the excessive release of growth factors (e.g., TGF-β and collagen types I and III), leading to an incremental increase in fibrotic tissue and muscle thickness. These growth factors could be a direct response to the provocative agents mediated by chronic injury or repair of airway epithelium but not directly dictated by the inflammatory response (Chapman, 2004, Churg et al., 2006, Gauldie

et al., 2002, Kenyon et al., 2003 and Selman et al., 2001). These findings suggest that inflammation and fibrosis may occur independently (Chapman, 2004, Gauldie et al., 2002 and Selman et al., 2001). Therefore, we reasoned that cigarette smoke exposure could cause opposite effects on airway inflammation, responsiveness and pulmonary remodeling in asthma. In the present study, we used an experimental model of allergic inflammation in BALB/c mice to investigate the Carfilzomib purchase effects of three weeks of mild cigarette smoke exposure on pulmonary inflammation and lung remodeling when both stimuli (i.e., allergen challenge and cigarette smoke) are administered simultaneously. Thirty-one male BALB/c mice (20–25 g) from the vivarium of the School of Medicine, University of Sao Paulo were divided into 4 groups as follows: animals non-sensitized and air-exposed (control group, n = 8); animals non-sensitized and exposed to cigarette smoke (CS group, n = 7); animals sensitized and air-exposed (OVA group, n = 7); and animals sensitized and exposed to cigarette smoke (OVA + CS Selleck Cobimetinib group, n = 9). This study was approved by the Review Board for Human and Animal Studies of the School of Medicine of the University of Sao Paulo.

All animal care and experimental procedures followed the EU Directive, 2010/63/EU for animal experiments guidelines ( Official Journal of the European Union, 2010). We used a modified OVA protocol from Vieira et al. (2007). BALB/c mice were sensitized by intraperitoneal (i.p.) injection of aluminum hydroxide-adsorbed ovalbumin (OVA) (50 μg per mouse) or saline (NaCl 0.9%) on days 0, 14 and 28. Twenty-one days after the first i.p. injection, mice were exposed to aerosolized OVA (1%) or saline 3 times per week for 30 min in the morning until day 42 (Fig. 1A). We used a modified cigarette smoke exposure protocol from Biselli et al. (2011) beginning 21 days after the first immunization and lasting until day 42, as in the OVA protocol.

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