We also thank Assoc Prof T Tsuge (Department of Innovative and

We also thank Assoc. Prof. T. Tsuge (Department of Innovative and engineered Materials, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, Japan) for GC-MS analysis. This work was supported by MEXT Grant-in-Aid for Scientific Research on Priority Areas “Applied Genomics” (Grant Number 20018008) and that on Innovative Areas “”Genome Science”" (Grant

Number 221S0002). Electronic supplementary material Additional file 1: Detection of phase-dependent transcriptomic changes and Rubisco-mediated CO 2 fixation into poly(3-hydroxybutyrate) under heterotrophic condition in CFTRinh-172 Ralstonia eutropha H16 based on RNA-seq and gene deletion analyses (Shimizu et al.). Figure S1. DMXAA nmr Relative expression changes of phaC1 determined by qRT-PCR using three primer sets for amplification and two inner control genes for quantification. Square, amplification of the central region (primers: phaC1-5’-Cent/phaC1-3’-Cent); diamond, amplification of the N-terminal region (phaC1-5’-N/phaC1-3’-N); circle, amplification of the C-terminal

region (phaC1-5’-C/phaC1-3’-C). Open symbols, bfr2 inner control; closed symbols, 16SrRNA inner control. Materials and Methods for qRT-PCR. Figure S2. Correlation of expression ratios from RNA-seq and qRT-PCR in F26. The best-fit linear regression curve is shown with the correlation coefficient (R2). Closed circle, dapA1 (primers: dapA1-5’/dapA1-3’); closed square, phaC1 (phaC1-5’-Cent/phaC1-3’-Cent); closed triangle, cbbL (cbbL-5’/cbbL-3’); closed diamond, bfr2 (bfr2-5’/bfr2-3’). The primer sequences are listed in Table S4, and qRT-PCR was performed as described in the legend of Figure S1. Table S1. Highly transcribed genes in R. euttopha H16 during the growth on fructose.a. Table S2. Highly up-regulated genes in F26 to F16. Table S3. Highly down-regulated genes in F26 to F16. Table S4. Primers used in this study. (PDF 1 MB) References 1. Bowien B,

Kusian B: Genetics next and control of CO 2 assimilation in the chemoautotroph Ralstonia eutropha . Arch Microbiol 2002, 178:85–93.PubMedCrossRef 2. Ishizaki A, Tanaka K, Taga N: Microbial production of poly-D-3-hydroxybutyrate from CO 2 . Appl Microbiol Biotechnol 2001, 57:6–12.PubMedCrossRef 3. find more Jendrossek D: Polyhydroxyalkanoate granules are complex subcellular organelles (carbonosomes). J Bacteriol 2009, 191:3195–3202.PubMedCrossRef 4. Rehm BHA: Polyester synthases: natural catalysts for plastics. Biochem J 2003, 376:15–33.PubMedCrossRef 5. Rehm BHA: Biogenesis of microbial polyhydroxyalkanoate granules: a platform technology for the production of tailor-made bioparticles. Curr Issues Mol Biol 2007, 9:41–62.PubMed 6. Steinbüchel A, Lütke-Eversloh T: Metabolic engineering and pathway construction for biotechnological production of relevant polyhydroxyalkanoates in microorganisms. Biochem Eng J 2003, 16:81–96.CrossRef 7.

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