We also thank Assoc. Prof. T. Tsuge (Department of Innovative and engineered Materials, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, Japan) for GC-MS analysis. This work was supported by MEXT Grant-in-Aid for Scientific Research on Priority Areas “Applied Genomics” (Grant Number 20018008) and that on Innovative Areas “”Genome Science”" (Grant
Number 221S0002). Electronic supplementary material Additional file 1: Detection of phase-dependent transcriptomic changes and Rubisco-mediated CO 2 fixation into poly(3-hydroxybutyrate) under heterotrophic condition in CFTRinh-172 Ralstonia eutropha H16 based on RNA-seq and gene deletion analyses (Shimizu et al.). Figure S1. DMXAA nmr Relative expression changes of phaC1 determined by qRT-PCR using three primer sets for amplification and two inner control genes for quantification. Square, amplification of the central region (primers: phaC1-5’-Cent/phaC1-3’-Cent); diamond, amplification of the N-terminal region (phaC1-5’-N/phaC1-3’-N); circle, amplification of the C-terminal
region (phaC1-5’-C/phaC1-3’-C). Open symbols, bfr2 inner control; closed symbols, 16SrRNA inner control. Materials and Methods for qRT-PCR. Figure S2. Correlation of expression ratios from RNA-seq and qRT-PCR in F26. The best-fit linear regression curve is shown with the correlation coefficient (R2). Closed circle, dapA1 (primers: dapA1-5’/dapA1-3’); closed square, phaC1 (phaC1-5’-Cent/phaC1-3’-Cent); closed triangle, cbbL (cbbL-5’/cbbL-3’); closed diamond, bfr2 (bfr2-5’/bfr2-3’). The primer sequences are listed in Table S4, and qRT-PCR was performed as described in the legend of Figure S1. Table S1. Highly transcribed genes in R. euttopha H16 during the growth on fructose.a. Table S2. Highly up-regulated genes in F26 to F16. Table S3. Highly down-regulated genes in F26 to F16. Table S4. Primers used in this study. (PDF 1 MB) References 1. Bowien B,
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