Cadeau for the correction of the manuscript. This work was supported by institutional grants from Copanlisib Inserm and the University of Angers and by grants from the Ligue contre le Cancer (Ligue nationale “Equipe labellisée 2012–2014” et les, Comités départementaux du Maine et Loire, de Loire Atlantique, de Sarthe et de Vendée), Cancéropole Grand-Ouest and Région Pays de la Loire (project CIMATH). U. Jarry was supported by the Association pour la Recherche contre le Cancer. The authors declare no financial or commercial conflict of interest. “
“Citation Sun Z, Jin F, Li Y, Zhang J. Immunocontraceptive effect of DNA
vaccine targeting fertilin β in male mice. Am J Reprod Immunol 2010; 63: 282–290 Problem In previous study, two eukaryotic expression plasmids pSG.SS.YL-Fβ.ECD and pSG.SS.C3d3.YL-Fβ.ECD were successfully constructed and transfected in HEK293 cells. Now, we want to evaluate the immunocontraceptive effect of these two DNA vaccines that target the extracellular domain (Fβ.ECD) of sperm antigen fertilin β subunit in Kunming
male mice. Method of study DNA vaccines pSG.SS.YL-Fβ.ECD and pSG.SS.C3d3.YL-Fβ.ECD were injected into Kunming male mice three times at 0, 4, and 8 weeks, respectively. An antifertility effect was observed. Serum antibody and cytokines were also detected. Results Both vaccines significantly decreased both the pregnancy Lumacaftor in vitro rate and the number of newborns. The serum levels of IL-2 and INF-γ significantly decreased, whereas the levels of IL-4 and IL-10 significantly increased. Compared with pSG.SS.YL-Fβ.ECD, 17-DMAG (Alvespimycin) HCl pSG.SS.C3d3.YL-Fβ.ECD was more effective in birth control, and its specific Fβ-IgG antibody titer in serum was significantly higher and longer. Conclusion The results indicate that both pSG.SS.YL-Fβ.ECD and pSG.SS.C3d3.YL-Fβ.ECD DNA vaccines are effective
in birth control of mice. The immunocontraceptive effect of Fβ.ECD DNA vaccine in male mice is improved with the addition of immuno-adjuvant C3d3. “
“Retinoic acid-inducible gene-I (RIG-I)-like receptors (RLR) are members of the DEAD box helicases, and recognize viral RNA in the cytoplasm, leading to IFN-β induction through the adaptor IFN-β promoter stimulator-1 (IPS-1) (also known as Cardif, mitochondrial antiviral signaling protein or virus-induced signaling adaptor). Since uninfected cells usually harbor a trace of RIG-I, other RNA-binding proteins may participate in assembling viral RNA into the IPS-1 pathway during the initial response to infection. We searched for proteins coupling with human IPS-1 by yeast two-hybrid and identified another DEAD (Asp-Glu-Ala-Asp) box helicase, DDX3 (DEAD/H BOX 3). DDX3 can bind viral RNA to join it in the IPS-1 complex. Unlike RIG-I, DDX3 was constitutively expressed in cells, and some fraction of DDX3 is colocalized with IPS-1 around mitochondria. The 622-662 a.