The Fr mortality increased significantly post-coppice. While Fr mortality was much lower than Fr productivity in 2011 (pre-coppice), it exceeded Fr productivity in 2012 (post-coppice). In both genotypes the average Fr biomass and necromass significantly declined with increasing soil depth (Fig. 4). Using MANOVA, was shown that all soil depths biomass of Fr in the first year of the second year (2012; post-coppice) XAV-939 cell line did not statistically differ from the second year of the first rotation (2011; pre-coppice). For

genotype Koster, however, Fr biomass in the upper soil layer increased in 2012 (post-coppice) as compared to 2011 (pre-coppice; Fig. 4) when the data was partitioned by depth. For genotype Skado, Fr biomass was higher in the former cropland than in the former pasture (Table

2). No genotypic differences in Fr biomass were detected Ipilimumab datasheet at any soil depth. The depth was a statistically significant factor in the MANOVA model. The highest Fr biomass was detected in the upper 15 cm. On average, Fr biomass in the upper 15 cm accounted for 63.6 ± 16.4 g DM m−2. The Fr biomass in the upper 15 cm of the soil represented 44.3% and 50.1% of the total Fr in the 0–60 cm profile of genotypes Skado and Koster, respectively. In the second year of the first rotation (2011; pre-coppice), Wr biomass, mostly from grasses, was significantly higher than Fr of poplar in the upper 45 cm of the root profile. Overall, in 2011 the Wr showed a strong vertical distribution with a significant concentration in the upper 30 cm, while in 2012 (post-coppice) the Wr were more evenly distributed over the soil profile than the Fr. For trees of the same BA, no significant differences in Cr biomass were detected, neither between genotypes nor between previous land-use types. Consequently one single allometric equation was established at each sampling campaign to scale-up Cr biomass of the two genotypes across both previous land-use types using the BA frequency distribution (Fig. 5). It was, however, not possible to establish an allometric equation for Mr (Fig. 5). The up-scaled standing

belowground woody biomass after both rotations significantly differed between both genotypes (Table 3). After the first rotation (pre-coppice), the Cr biomass was already higher in Skado (145.9 g DM m−2) many than in Koster (95.3 g DM m−2). After coppice, the Cr biomass increased by 28% and by 63% to 187 g DM m−2 and 155 g DM m−2 for Skado and Koster, respectively Table 3. The C concentration of the roots increased with root diameter class (Fr, Mr and Cr, Table 4). The C concentration was lowest (36% of C) in the Fr without significant differences between necromass and biomass. There were no significant genotypic differences in root C concentration. After the first rotation, most of the C was stored in the Cr, with 53.5 g C m−2, followed by the Fr 40.1 g C m−2 and Mr 35.3 g C m−2.