In contrast, CusCFBA had a narrow substrate spectrum, transporting Cu(I) and Ag(I) almost exclusively. Three conserved residues in these metal exporters might be responsible for substrate recognition and specificity.
We greatly appreciate our colleagues for the supply of the strains and plasmids. Helen Zgurskaya provided the E. coli deletion strains W4680AD and W4680AE, as well as valuable correspondence. The deletion strain E. coli 5X RND and plasmid pCusCFBA were supplied by Dietrich Nies. Fernando Soncini provided plasmids pUH21 and pGesAB. E.H.-K. is a scholar of the Alfred P. Sloan Foundation. This work was supported by National Institutes of Health AZD2014 Grant GM079192 to M.M.M. and C.R. Fig. S1. Biolog plots for dinitrobenzene (top), dinitrophenol (middle), and ethionamide (bottom). The absorbance of the reduced tetrazolium dye was plotted versus time of exposure. E. coli strain W4680AD containing pCusCFBA (dashed) grew at a faster rate than the E. coli strain W4680AD containing the control vector pGEM-T (solid) for all three selleck compound chemicals. Fig. S2. Growth of Escherichia coli strains W4680AD (top), W4680AE (middle), and 5X RND (bottom) expressing pCusCFBA (dashed line) or the control vector pGem-T (solid line) in liquid media containing different concentrations of dinitrobenzene. Fig. S3. Biolog results for chlorquinaldol
(top), chloramphenicol (middle), and dichlofluanid (bottom). E. coli strain W4680AD containing pGesAB (dashed) grew at a faster rate than the E. coli strain W4680AD containing the control vector pUH21(solid) for all three chemicals. Fig. S4. Growth of Escherichia coli strains W4680AD (top), W4680AE (middle), and 5X RND (bottom) harboring pGesAB (dashed line) or pUH21 (solid line) in liquid media containing different concentrations of crystal violet. Table S1. Chemicals in the Biolog Chemical Sensitivity Panels PM11–PM20. Please
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“Escherichia coli are enteric Gram-negative bacilli that can colonize the female genital tract and become implicated in different infections in pregnant women, including intra-amniotic infection, puerperal infections and neonatal infections. The virulence profiles of E. coli isolates from vaginal swabs from pregnant and nonpregnant women were compared. The hly-, cnf-, pap- and iroN-genes were found significantly more frequently in E. coli isolated from pregnant women in comparison with those isolated from nonpregnant women. Escherichia coli from pregnant women seem to be more virulent than from nonpregnant women developing severe infections, thereby increasing possible neonatal sepsis. Escherichia coli are enteric Gram-negative bacilli found most frequently in the genital tract of women.