05) The regenerates in the control group and stretched regenerat

05). The regenerates in the control group and stretched regenerate areas of the ARM groups were similar regarding new bone formation (P > .05).

Conclusion. Acute regenerate molding could be safely applied immediately after the distraction period to correct postdistraction deformities without compromising bone healing. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010; 110: 554-559)”
“We

consider a one-dimensional twisted photonic liquid crystal, which consists of N nematic liquid crystal slabs in a twisted configuration alternated by N isotropic dielectric layers under the action of a dc electric field (E(dc)) aligned along the periodicity axis. We write and solve numerically the corresponding click here Euler-Lagrange equations describing the nematic layer configuration. We express Maxwell’s equations in a 4 x 4 matrix representation, and by using the transfer matrix formalism, we obtain the optical

band structures at arbitrary incidence angles and different external electric fields. We have found that there exists a strong dependence of electric field on the transmission and reflection spectra in enhancing and extinguishing bandgaps. The analysis presented here allow us to propose an electrically shiftable universal rejection filter for incident waves of left-and right-circular polarization. It is observed that by increasing the electric field we can highly enhance the cross-polarized reflection bandgaps and suppress the co-polarized ones. We analyzed the optical spectra for different values of twist angle, different ratios between dielectric and nematic layer thicknesses Mocetinostat in vitro and number of layers N. Also, we showed that the cross-polarized

bandgaps are blue-shifted as the incidence angle gets larger. (C) 2011 American Institute of Physics. [doi: 10.1063/1.3575154]“
“DNA extraction is an essential step for molecular analysis of an organism, but it is difficult to acquire a sufficient amount of pure DNA from plant tissue with high levels of phenolic compounds, carbohydrates, proteins, and secondary metabolites. Jerusalem artichoke (Helianthus tuberosus) has high levels of such substances. We compared five commonly used methods of extracting genomic DNA in tests made with leaves and seed of four PD0325901 manufacturer Jerusalem artichoke genotypes: 1) modified method of Tai and Tanksley, 2) method of Doyle and Doyle, 3) method of Porebski, 4) modified method of Storchova, and 5) Plant DNA Kit of Omega Bio-tek. The quality and quantity of extracted DNAs were assessed by photometric assay, electrophoresis on 1% agarose gel and a PCR-based technique. The modified method of Tai and Tanksley was found to be superior for both young leaves and seed. The quality of the extracted DNA was confirmed by sequence-related amplified polymorphism. This information will be useful for molecular analyses of Jerusalem artichoke and other related Helianthus species.”
“Objective.

Methods: We investigated whether the use or non-use of statins in

Methods: We investigated whether the use or non-use of statins influenced the prevalence of airflow limitation. All outpatients who were over the age of 40 years and who regularly visited a primary health care facility were invited to participate. Each participant underwent spirometry and completed a questionnaire regarding their clinical status, which was used to screen for COPD. A variety of factors that are potentially

related to airflow limitation were assessed.

Results: Of the 853 patients included in the study, 81 (9.5%) had airflow limitation. The prevalence of airflow limitation was 2.3% among the 89 patients with a history of statin use, which was five times lower than the prevalence

of airflow limitation among patients who had not used statins (10.5%). Among the 347 patients with a history of past or current smoking, airflow limitation was Stem Cell Compound Library not observed in the 30 patients who had used statins. However, by multivariate analysis, statin use was not significantly associated with a lower prevalence of airflow limitation.

Conclusions: This is the first cross-sectional study from Japan that has demonstrated that statin use has a potential impact on airflow limitation in patients with COPD.”
“Assessment of applicability is an essential part of the systematic review process. In the context of systematic reviews of the effects of interventions, applicability is an assessment of whether the findings of a review can be applied in a particular context www.selleckchem.com/products/Tipifarnib(R115777).html or population. For more complex interventions, assessing applicability can be challenging because of greater diversity of, and interactions within and between, the intended population, intervention components, comparison conditions, and outcomes as well as a range of further HSP inhibitor review considerations related to intervention context and theoretical basis. We recommend that review authors plan and conduct analyses to explain variations in effect and answer questions about mechanisms of action and influence of different settings, contexts, and populations. We also recommend that review authors provide rich descriptions of the setting,

implementation details, resource use, and contexts of included studies and assess applicability for at least one target population, setting, and context. This should facilitate applicability assessments by end users. Consensus on terminology is needed and guidance should be developed for the synthesis of implementation information within reviews as well as the documentation of applicability judgments by review authors. (c) 2013 Elsevier Inc. All rights reserved.”
“SETTING: Contact investigation resulting from specimens sent to the Scottish Mycobacteria Reference Laboratory.

OBJECTIVE: To characterise patients and types of exposures associated with transmission of a prevalent Mycobacterium tuberculosis genotype in Scotland.


“This report describes the pathologic findings for a patie


“This report describes the pathologic findings for a patient BAY 73-4506 concentration with Hunter syndrome who underwent aortic valve replacement at 10 years of age, 3 years after the initiation of enzyme replacement therapy. Aortic valve pathology showed mild thickening and fibrosis as well as massive glycosaminoglycan accumulation. This suggests that enzyme replacement therapy has limited efficacy for cardiac valve disease both clinically and pathologically.”
“Objectives.

The objective of this study was to observe the efficacy, safety, and side effects of a combination of flunarizine plus topiramate compared with either flunarizine and or toparamate alone for migraine prophylaxis.

Methods. Out of 150 patients with migraine recruited into the study and randomly assigned to one of three conditions, 126 completed

the trial in their group: flunarizine (39), topiramate (44), and flunarizine plus topiramate (43). Patient information was assessed at enrollment and at follow-up visits at the end of months 1-3, 6, 9, and 12. The primary measure of efficacy reduction in mean monthly migraine frequency of at least 50% as compared with baseline. Secondary efficacy Proteases inhibitor parameters included reduction in mean monthly migraine days and severity of headache. Side effects were compared in the three groups by recording adverse reactions and weight changes.

Results. The proportion whose monthly headache frequency decreased more than 50% was 66.7% (26/39) in the flunarizine group, 72.7% (32/44) in the topiramate group and 76.7% (33/43) in the combination group, respectively (P = 0.593). The mean monthly days and severity of headache in the three groups also declined and was more significant in the flunarizine plus topiramate group than in the flunarizine group and the topiramate selleck products group (P < 0.05). In the flunarizine group, the average

weight change was 0.6 kg. Topiramate was associated with a mean weight loss was of -0.9 kg in the topiramate group and -0.2 kg in the flunarizine plus topiramate group.

Conclusion. Flunarizine, topiramate, and the combination of flunarizine with topiramate are all effective and have good tolerability in migraine prophylaxis. Adding topiramate to flunarizine may reduce the latter’s impact on body weight.”
“Coarctation of the abdominal aorta is extremely rare. It generally involves a long segment of the descending aorta and causes uncontrolled and unexplainable hypertension in children. The therapeutic choice is very challenging because acute and chronic complications are reported for both the surgical and the percutaneous approaches. The two reported cases of abdominal coarctation were treated primarily and successfully through the use of covered stents. Three covered stents were implanted in two children. No complication occurred with either procedure.

Here, we describe a case of a 13-year-old girl with idiopathic in

Here, we describe a case of a 13-year-old girl with idiopathic intracranial

hypertension refractory to long-term trials of acetazolamide and furosemide at maximally tolerated doses. After declining surgical intervention despite progression of her visual symptoms, a trial of bumetanide (0.25 mg daily) monotherapy was successful in resolution of the patient’s symptoms. These results suggest bumetanide could be effective in the treatment of idiopathic intracranial hypertension, perhaps by restoring the balance between cerebrospinal fluid formation and absorption and/or by altering the volume or ionic composition of the brain’s extracellular fluid compartment.”
“Objective: To examine the relationship between prepotent inhibition capacities and cigarette dependence in a sample of non-deprived light to moderate smokers.

Methods: Fifty volunteer smokers were screened with Caspase inhibitor clinical trial a laboratory go-stop paradigm, and self-reports of cigarette dependence (Fagerstrom Test for Nicotine Dependence, FTND) and cigarette craving (revised Questionnaire on Smoking Urge,

QSU-12).

Results: Correlation and regression analyses showed that lower prepotent inhibition capacities predict higher Selleckchem Silmitasertib levels of cigarette dependence when individual differences in processing speed, craving states, and age were controlled for. In addition, lower inhibition capacity is associated with a higher number of cigarettes smoked per day.

Conclusions: A poor ability to inhibit prepotent click here responses seems to be one of the individual factors related to cigarette smoking dependence. (C) 2010 Elsevier Ireland Ltd. All rights reserved.”
“Purpose: To study the interrelationship between patella cartilage T2 relaxation time, other knee abnormalities, and physical

activity levels in asymptomatic subjects from the Osteoarthritis Initiative (OAI) incidence cohort.

Materials and Methods: The study had institutional review board approval and was HIPAA compliant. One hundred twenty subjects from the OAI without knee pain ( age, 45-55 years) and with risk factors for knee osteoarthritis (OA) were studied by using knee radiographs, 3.0-T knee magnetic resonance (MR) images ( including intermediate-weighted fast spin-echo and T2 mapping sequences), and the Physical Activity Scale for the Elderly. MR images of the right knee were assessed by two musculoskeletal radiologists for the presence and grade of abnormalities. Segmentation of the patella cartilage was performed, and T2 maps were generated. Statistical significance was determined by using analysis of variance, chi(2) analysis, correlation coefficient tests, the Cohen kappa, and a multiple linear regression model.

Results: Cartilage lesions were found in 95 (79.0%) of 120 knees, and meniscal lesions were found in 54 (45%) of 120 knees. A significant correlation between patella cartilage T2 values and the severity and grade of cartilage (P = .0025) and meniscus (P = .0067) lesions was demonstrated.

Once placed, each skin staple position was marked for later refer

Once placed, each skin staple position was marked for later referencing. A surface temperature laser device recorded prescan skin surface temperature. A 35-minute MRI scan was performed with a 1.5-Tesla magnet and standard knee coil for each pig foot. Immediately afterward, the skin surface temperature and displacement measurements were recorded. The paired t test was used to analyze temperature change from prescan to postscan.

Results: The prescan mean temperature was 16.45 degrees C (standard deviation: 0.70 degrees C), and the range was 14.60 degrees

C to 18.20 degrees C. After scanning, the mean temperature was 16.02 degrees C (standard deviation: 0.63 degrees C), and the range was 15.00 degrees C to 17.60 degrees C. The decrease of 0.43 degrees C in skin surface temperature was statistically significant (p = 0.001). No selleck change in staple position was measurable or evident by visual inspection for any of the pig feet.

Conclusion: This study found no increase in skin surface temperature or displacement of staple position after a standard extremity MRI scan. Based on our findings, MRI scanning in the presence of stainless steel surgical staples seems safe.”
“Combination of ruptured sinus of Valsalva aneurysm

(SVA), and a coexisting asymptomatic adult aortic isthmic coarctation is extremely rare. The timing and sequence of surgical and/or AZD2014 ic50 interventional repair of these two pathologies are controversial. We present a case of a 37-year-old male who was admitted to our department because of severe acute congestive heart failure and signs of ruptured aneurysm of the SV into the right ventricle. Transthoracic and transoesophageal echocardiography confirmed the communication between an important right coronary SVA and right ventricle, bicuspid aortic valve, mild aortic regurgitation, and revealed ACY-241 mouse severe aortic coarctation. Because of the severe dilation of right sinus of Valsalva a surgical repair of the ruptured aneurysm was performed. Aortic coarctation was treated four

weeks later by a percutaneous stent-graft implantation. This case report supports the concept that hybrid approach is feasible in patients with ruptured SVA and aortic coarctation in adulthood. (C) 2011 Published by European Association for Cardio-Thoracic Surgery. All rights reserved.”
“Changes in diet and lifestyle in recent years have led to unhealthy dietary patterns and inadequate physical activity, making it difficult to maintain an appropriate energy balance, which results in an increased prevalence of diet-related chronic diseases such as obesity, diabetes, cardiovascular disease, and certain types of cancer. The importance of the roles of lipids in these diseases is now recognized. Dietary fatty acids modulate inflammatory processes and contribute to the pathophysiological state of diet-related chronic diseases.

For

other metals, the initial blank tests showed possible

For

other metals, the initial blank tests showed possible contamination of sampling systems, and really pointed to the importance of blank sampling.

Finally, this trial highlighted the need for general guidelines for sampling that could minimize the risk of misclassification of lake-water status. (C) 2012 Elsevier Ltd. All rights reserved.”
“A new source of lipase from Bacillus sp. ITP-001 was immobilized by physical adsorption on the polymer poly(3-hydroxybutyrate-co-hydroxyvalerate) (PHBV) in aqueous solution. The support and immobilized lipase were characterised, compared to the lyophilised lipase, with regard to the specific surface area, adsorption-desorption isotherms, pore volume (Vp) and size (dp) by nitrogen adsorption, differential scanning calorimetry, thermogravimetric Histone Methyltransf inhibitor analysis, chemical composition analysis, Fourier transform infrared spectroscopy and biochemical properties. The immobilized enzyme displayed a shift in optimum pH towards the acidic side AZD1480 with an optimum at pH 4.0, whereas the optimum pH for the free enzyme was at pH 7.0; the optimum temperature of activity was 80 and 37 A degrees C for the free and immobilized enzyme, respectively.

The inactivation rate constant for the immobilized enzyme at 37 A degrees C was 0.0038 h(-1) and the half-life was 182.41 h. The kinetic parameters obtained for the immobilized enzyme gave a Michaelis-Menten constant (K (m)) of 49.10 mM and a maximum reaction velocity (V (max)) of 205.03 U/g. Furthermore, the reuse of the lipase immobilized by adsorption allowed us to observe that it could be reused for 10 successive cycles, duration of each cycle (1 h), maintaining 33 % of the initial activity.”
“OBJECTIVE: To examine the effects and safety of high-dose (compared with low-dose) oxytocin regimen for labor augmentation on perinatal outcomes.

METHODS: Data from the Consortium on Safe Labor were used. A total of 15,054 women from six hospitals were eligible for the analysis. Women were grouped based on their oxytocin starting dose and incremental dosing of 1, 2, and 4 milliunits/min. Duration of labor and a number of maternal and neonatal outcomes

were compared among these three groups stratified by parity. Multivariable logistic regression and generalized GSI-IX price linear mixed model were used to adjust for potential confounders.

RESULTS: Oxytocin regimen did not affect the rate of cesarean delivery or other perinatal outcomes. Compared with 1 milliunit/min, the regimens starting with 2 milliunits/min and 4 milliunits/min reduced the duration of first stage by 0.8 hours (95% confidence interval 0.5-1.1) and 1.3 hours (1.0-1.7), respectively, in nulliparous women. No effect was observed on the second stage of labor. Similar patterns were observed in multiparous women. High-dose regimen was associated with a reduced risk of meconium stain, chorioamnionitis, and newborn fever in multiparous women.

085 g 1-butanol/g zeolite (9 3 min residence time) A subsequent

085 g 1-butanol/g zeolite (9.3 min residence time). A subsequent temperature swing leads to desorption. By choosing the temperature

program carefully, it was possible to separate the water/ethanol/acetone and 1-butanol fractions. The resulting I-butanol concentration in the 1-butanol fraction was 84.3 wt% and thus a concentration factor of 65 was achieved in one step, which is a higher value compared to other isolation techniques. Only 80% of adsorbed 1-butanol could be recovered, the remainder could only be desorbed at higher temperatures as butene. However, this should not be a problem in an industrial Entinostat supplier process as all stronger binding, catalytic sites will be blocked after the first adsorption/desorption round. A mathematical model was developed to simulate the breakthrough data and a mass transfer coefficient (k(p)a) of 0.052 min(-1) was obtained. Comparison of simulated kpa for different sizes of extrudates clearly indicated that the adsorption rate is determined by solid phase diffusion. (C) 2009 Elsevier B.V. All rights reserved.”
“Persistent oxidative stress has been associated with carcinogenesis. Iron NVP-AUY922 clinical trial overload is considered one such condition that causes oxidative stress. Epidemiological studies support a close

link between iron overload and carcinogenesis. Reportedly, regular semiannual phlebotomies reduced cancer risk in an otherwise normal population. More specifically, genetic hemochromatosis, chronic viral hepatitis, ovarian endometriosis and asbestosis induce iron overload, which can lead to hepatocellular carcinoma, ovarian carcinoma CAL-101 research buy or mesothelioma in humans. Through a combination of animal experiments and microarray analyses, homozygous deletion of CDKN2A/2B has been recognized

as one of the major target genes involved in iron overload-induced carcinogenesis. CDKN2A/2B are the second most frequently inactivated tumor suppressing genes in human cancers. Currently, when infection is becoming sufficiently controlled worldwide, iron regulation may be the next target for human longevity.”
“The aim of this work was to study the effect of crystallinity degree on the thermal, mechanical, and fracture properties of poly(lactic acid)/organomodified montmorillonite (PLA/OMMT) nanocomposites. Samples with two different compositions (0.5 and 2.5% weight OMMT in PLA) were prepared by melt mixing in a twin-screw extruder and injection molding. An annealing treatment was applied to increase the percentage of PLA crystallinity. The thermal behavior was analyzed by differential scanning calorimetry (DSC), and the mechanical properties were determined via tensile tests and the fracture behavior using the linear elastic fracture mechanics theory, using SENB specimens at low and high testing rates. Gel permeation chromatography (GPC) from granulates and injected specimens were also carried out, finding some polymer degradation during extrusion and injection processes.

The lethal dose, 50% (LD50) of per oral geniposide was 1431 1mgkg

The lethal dose, 50% (LD50) of per oral geniposide was 1431.1mgkg(-1). mTOR kinase assay The acute toxicity study indicated geniposide at dose of 574mgkg(-1) or more could cause hepatic toxicity in rats and the hepatotoxicity often appeared at 24-48h

after the oral administration. The hepatotoxicity was associated with oxidative stress with decrease of total superoxide dismutase activity and increase of malondialdehyde concentration in rats’ livers. Subchronic toxicity study showed geniposide did not cause hepatotoxicity at the doses of 24.3 and 72.9mgkg(-1) orally for 90 days in rats. Thus, acute hepatotoxicity of geniposide at high doses was likely to be linked to oxidative stress, while geniposide at normal dose of 24.3mgkg(-1) or less did not cause hepatotoxicity even in the repeated dosing study.”
“Background:

Sexual transmission is the fastest growing route of HIV transmission in China. We undertook this study to describe the risk factors for HIV infection in female sex workers (FSWs), and to determine the commercial sex venues where FSWs are most at risk of being infected with or infecting others with HIV.

Methods: This was a cross-sectional study of 737 FSWs in Kaiyuan City, Yunnan Province in southern China, which took place from March to May 2006.

Results: The overall HIV prevalence was 10.3%, but prevalence varied with sex venue with 25.8% of FSWs working on the streets being HIV-positive and none of the FSWs working in nightclubs. Adjusted odds ratios (OR) of HIV infection learn more were 9.1 (95% confidence interval (CI) 4.67-17.55) for injection drug use, 3.3 (95% CI 1.46-7.37) for non-injection illegal drug use, 2.7 (95% CI 1.25-5.93) for duration of sex work >= 5 years, 2.2 (95% CI 1.05-4.70) for infection with herpes simplex virus type 2, and 2.0 (95% 8-Bromo-cAMP solubility dmso CI 1.12-3.47) for working at a higher risk entertainment venue. Although condom use was not a significant risk factor in the overall model, FSWs in tower risk venues who reported consistent use with clients had a 70% reduction in HIV infections (OR 0.30, 95% CI 0.12-0.90).

Conclusions: Illegal drug use, particularly with injection drugs, is the single greatest risk

factor for HIV infection among FSWs in Kaiyuan City, China. FSWs working on the street or in temporary sublets, beauty salons, or saunas are at particularly high risk for transmitting and being infected with HIV. HIV prevention efforts among FSWs should target illegal drug users and these other subgroups. (C) 2008 International Society for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.”
“The objective of this study was to investigate invitro antimicrobial activity and composition of the essential oil of Cyclotrichium leucotrichum growing wild in Iran. The essential oil was obtained by hydro-distillation and analysed by GC-FID and GC/MS. Fifty-nine components representing 98.9% of the total oil were characterised.

This kind of headache is recognized

as a biobehavioral di

This kind of headache is recognized

as a biobehavioral disorder, a complex condition wherein emotion and pain are intermingled. Daporinad Properly treating medication overuse with adequate strategies by different and specific steps are essential components for helping these patients to improve and for preventing relapses. The clinical experience performed at our Headache Unit, the necessity of withdrawal, different treatment schedules, and different treatment strategies of our center were discussed.”
“The effect of three different alkylammonium-modified montmorillonite on morphological and mechanical properties of glassy epoxy-amine nanocomposites is reported. Small amounts of clays <10 phr (part per hundred of resin) were used in each system of nanocomposite. The morphology of the prepared nanocomposites was performed by means of X-ray diffraction and transmission electron microscopy. Differential scanning calorimetry (DSC) was used to investigate the glass transition temperatures (Tg). Mechanical properties were based on tensile characteristics (Young’s modulus), see more impact strength, and fracture toughness. The measured moduli were compared to theoretical predictions. Scanning electron microscopy was used to study the morphological structure of the fracture surfaces of impacted specimens. It was found that at a low content of 2 phr (1.2 wt %) of nanoclays, the impact strength and the fracture toughness were improved by 77 and 90% respectively, comparatively

to the neat epoxy, whereas DSC revealed a reduction of the Tg of nanocomposites. (c) 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2011″
“Although the quiescent center (QC) is crucial to root development, the molecular mechanisms that regulate its postembryonic maintenance remain obscure. In this study, a semi-dominant mutant that exhibits pleiotropic defects in root tissues, which includes the root cap, lateral and crown roots, was isolated. The mutant is characterized by a loss of QC identity during postembryonic development, and the displayed defects result from a stabilizing mutation in domain II of OsIAA23 (Os06g39590). Expression of OsIAA23 is specific

to the QC of the root tip during the development of primary, lateral and crown roots. Consistent with OsIAA23 expression in this website the QC, the auxin signaling marked by DR5p::GUS (beta-glucuronidase) was absent in the QC region of Osiaa23. Transgenic rice plants harboring Osiaa23 under the control of the QHB promoter mimic partially the defects of Osiaa23. These results indicate that the maintenance of the QC is dependent on OsIAA23-mediated auxin signaling in the QC. These findings provide insight into Aux/IAA-based auxin signaling during postembryonic maintenance of the QC in plants.”
“The authors investigated the possibility of improving positioning of stimulation leads in patients with chronic neuropathic peripheral nerve pain and good pain relief from implantation of a peripheral nerve stimulator (PNS).

5)Te(0 5) (C) 2011 American Institute

of Physics [doi:1

5)Te(0.5). (C) 2011 American Institute

of Physics. [doi:10.1063/1.3624759]“
“A novel charring agent (CNCA-DA) containing triazine and benzene ring, using cyanuric chloride, aniline, and ethylenediamine as raw materials, was synthesized and characterized. The effects of CNCA-DA on flame retardancy, thermal degradation, and flammability properties of polypropylene (PP) were investigated by limited oxygen index (LOI), vertical burning test (UL-94), thermogravimetric analysis (TGA), and cone calorimeter test (CCT). The TGA results showed that CNCA-DA had a good char forming ability, and a high find more initial temperature of thermal degradation; the char residue of CNCA-DA reached 18.5% at 800 degrees C; Ammonium polyphosphate (APP) could improve the char residue of APP/CNCA-DA system, the char residue reached 31.6% at 800 degrees C. The results from LOI and UL-94 showed that the intumescent flame retardant (IFR) containing CNCA-DA and APP was very effective in flame retardancy of PP. When the mass ratio of APP and CNCA-DA was 2 : 1, and the IFR loading was 30%, the IFR showed the best effect; the LOI value reached 35.6%. It was also found that when the IFR loading

was only 20%, the flame retardancy of PP/IFR can still pass V-0 rating in UL-94 tests, and its LOI value reached 27.1%. The CCT results demonstrated that IFR Oligomycin A supplier could clearly change the decomposition behavior of check details PP and form a char layer on the surface of the composites, consequently resulting in efficient reduction of the flammability parameters, such as heat release rate (HRR), total heat release (THR), smoke production rate (SPR), total smoke production (TSP), and mass loss (ML). (C) 2011 Wiley Periodicals, Inc. J Appl Polym Sci 123: 3208-3216, 2012″
“One of the WHSC1/MMSET/NSD2 variant RE-IIBP is a histone H3-K27 methyltransferase with transcriptional repression activity.

Overexpression of RE-IIBP in various types of leukemia suggests it’s role in leukemogenesis. Here we identify two proteins, KRAB zinc finger protein ZNF 350 and enolase-1 as RE-IIBP interacting proteins by yeast two-hybrid screening and confirmed direct interaction in vivo and in vitro. Both proteins have been known for their role in transcriptional repression. Reporter assays using transient transfection demonstrated that both ZNF 350 and enolase-1 proteins synergistically repressed transcription with RE-IIBP, respectively. These results indicate both proteins have roles in RE-IIBP mediated transcriptional repression by involving co-repressor complex.”
“The magnetization structure and the mechanisms of magnetic interaction in an artificial two-phase magnet are analyzed with magnetic force microscopy and magnetooptics. A model system built up of arrays of hard magnetic CoPt squares (5 mu m edge length) embedded in a soft magnetic permalloy matrix is investigated.